Part:BBa_K2623007:Design

SAHS coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 218
  Illegal XhoI site found at 442
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 497
  Illegal SapI.rc site found at 492
  

Design Notes

We got the DNA sequence of SAHS from DDBJ. And then we did some codon optimization to the DNA sequence for E.coli (strain K12).

Source

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) SAHS gene.